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Dr Diane Proudfoot

Dr Diane Proudfoot

Senior Research Scientist

BHF Career Re-entry Fellow


Research Interests

My research focuses on the development of bone-like calcium phosphate particles in blood vessels, that occurs during ageing and in diseases such as atherosclerosis and hypertension. In particular, very small particles of calcium phosphate are associated with vulnerable atherosclerotic plaques. My studies in human vascular smooth muscle cells found that micro- and nanoparticles of calcium phosphate induced calcium signalling and cell death. Death of vascular smooth muscle cells weakens atherosclerotic plaques and causes further calcification and stimulation of pro-inflammatory pathways.  Investigating the mechanisms involved in calcium phosphate-induced cell death and inflammation may lead to therapeutic strategies to prevent the problems associated with calcification in ageing and cardiovascular disease.

Keywords

vascular smooth muscle cells (VSMC) ; Cell Death ; Atherosclerosis ; calcium signalling ; Vascular calcification ; cell signalling ; Inflammation ; Calcium Phosphate Particles ; cell death

Collaborators

Collaborators outside this directory

Key Publications

Dautova Y, Kozlova D, Skepper JN, Epple M, Bootman MD, Proudfoot D.  Fetuin-A and albumin alter cytotoxic effects of calcium phosphate nanoparticles on human vascular smooth muscle cells. PLoS One. 2014 May 21;9(5):e97565. doi: 10.1371/journal.pone.0097565.

Proudfoot D, Shanahan CM. Nanocrystals seed calcification in more ways than one. Kidney Int. 2011 Feb;79(4):379-82. doi: 10.1038/ki.2010.455.

Fetuin-A and albumin alter cytotoxic effects of calcium phosphate nanoparticles on human vascular smooth muscle cells. Dautova Y, Kozlova D, Skepper JN, Epple M, Bootman MD, Proudfoot D. PLoS One. 2014 May 21;9(5):e97565.

Human vascular smooth muscle cell culture. Proudfoot D, Shanahan C. Methods Mol Biol. 2012;806:251-63.

Calcium phosphate crystals induce cell death in human vascular smooth muscle cells: a potential mechanism in atherosclerotic plaque destabilization. Ewence AE, Bootman M, Roderick HL, Skepper JN, McCarthy G, Epple M, Neumann M, Shanahan CM, Proudfoot D. Circ Res. 2008 Aug 29;103(5):e28-34.

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