Research
Transcriptional Regulation of Blood Stem cells
My group uses a combination of experimental and computational approaches to study how transcription factor networks control the function of blood stem cells and how mutations that perturb such networks cause leukaemia. This integrated approach has resulted in the discovery of new combinatorial interactions between key blood stem cell regulators, as well as experimentally validated computational models for blood stem cells.
Current research focuses on
- single cell genomics of early cardiovascular development
- computer models to chart the transcriptional landscape of blood stem and progenitor cell differentiation
- transcriptional consequences of leukaemogenic mutations in leukaemia stem/progenitor cells
- molecular characterisation of human blood stem/progenitor cell populations used in cell and gene therapy protocols.
Publications
Please follow the link to PubMed for a full list of publications.
Defining the earliest step of cardiovascular lineage segregation by single-cell RNA-seq. Lescroart F, Wang X, Lin X, Swedlund B, Gargouri S, Sànchez-Dànes A, Moignard V, Dubois C, Paulissen C, Kinston S, Göttgens B, Blanpain C. Science. 2018 Jan 25.
From haematopoietic stem cells to complex differentiation landscapes. Laurenti E, Göttgens B. Nature. 2018 Jan 24;553(7689):418-426.
Defining murine organogenesis at single-cell resolution reveals a role for the leukotriene pathway in regulating blood progenitor formation. Ibarra-Soria X, Jawaid W, Pijuan-Sala B, Ladopoulos V, Scialdone A, Jörg DJ, Tyser RCV, Calero-Nieto FJ, Mulas C, Nichols J, Vallier L, Srinivas S, Simons BD, Göttgens B, Marioni JC. Nat Cell Biol. 2018 Feb;20(2):127-134.
The Human Cell Atlas. Regev A, Teichmann SA, Lander ES, Amit I, Benoist C, Birney E, Bodenmiller B, Campbell P, Carninci P, Clatworthy M, Clevers H, Deplancke B, Dunham I, Eberwine J, Eils R, Enard W, Farmer A, Fugger L, Göttgens B, Hacohen N, Haniffa M, Hemberg M, Kim S, Klenerman P, Kriegstein A, Lein E, Linnarsson S, Lundberg E, Lundeberg J, Majumder P, Marioni JC, Merad M, Mhlanga M, Nawijn M, Netea M, Nolan G, Pe'er D, Phillipakis A, Ponting CP, Quake S, Reik W, Rozenblatt-Rosen O, Sanes J, Satija R, Schumacher TN, Shalek A, Shapiro E, Sharma P, Shin JW, Stegle O, Stratton M, Stubbington MJT, Theis FJ, Uhlen M, van Oudenaarden A, Wagner A, Watt F, Weissman J, Wold B, Xavier R, Yosef N; Human Cell Atlas Meeting Participants. Elife. 2017 Dec 5;6.
Single-cell analysis reveals the continuum of human lympho-myeloid progenitor cells. Karamitros D, Stoilova B, Aboukhalil Z, Hamey F, Reinisch A, Samitsch M, Quek L, Otto G, Repapi E, Doondeea J, Usukhbayar B, Calvo J, Taylor S, Goardon N, Six E, Pflumio F, Porcher C, Majeti R, Göttgens B, Vyas P. Nat Immunol. 2018 Jan;19(1):85-97.
Critical Modulation of Hematopoietic Lineage Fate by Hepatic Leukemia Factor. Wahlestedt M, Ladopoulos V, Hidalgo I, Sanchez Castillo M, Hannah R, Säwén P, Wan H, Dudenhöffer-Pfeifer M, Magnusson M, Norddahl GL, Göttgens B, Bryder D. Cell Rep. 2017 Nov 21;21(8):2251-2263.
Single-cell RNA-sequencing reveals a distinct population of proglucagon-expressing cells specific to the mouse upper small intestine. Glass LL, Calero-Nieto FJ, Jawaid W, Larraufie P, Kay RG, Göttgens B, Reimann F, Gribble FM. Mol Metab. 2017 Oct;6(10):1296-1303.
Assessing the reliability of spike-in normalization for analyses of single-cell RNA sequencing data. Lun ATL, Calero-Nieto FJ, Haim-Vilmovsky L, Göttgens B, Marioni JC. Genome Res. 2017 Nov;27(11):1795-1806.
Establishment of mouse expanded potential stem cells. Yang J, Ryan DJ, Wang W, Tsang JC, Lan G, Masaki H, Gao X, Antunes L, Yu Y, Zhu Z, Wang J, Kolodziejczyk AA, Campos LS, Wang C, Yang F, Zhong Z, Fu B, Eckersley-Maslin MA, Woods M, Tanaka Y, Chen X, Wilkinson AC, Bussell J, White J, Ramirez-Solis R, Reik W, Göttgens B, Teichmann SA, Tam PPL, Nakauchi H, Zou X, Lu L, Liu P. Nature. 2017 Oct 19;550(7676):393-397.
Reconstructing blood stem cell regulatory network models from single-cell molecular profiles. Hamey FK, Nestorowa S, Kinston SJ, Kent DG, Wilson NK, Göttgens B. Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):5822-5829.
