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Cambridge Cardiovascular

 

Research

Calcification: My research focuses on the development of bone-like calcium phosphate particles in blood vessels, that occurs during ageing and in diseases such as atherosclerosis, hypertension and chronic kidney disease. My studies in human vascular smooth muscle cells found that micro- and nanoparticles of calcium phosphate have damaging properties by inducing calcium signalling, pro-inflammatory signalling and cell death. Investigating the nature, location and activity of calcium phosphate particles could aid our understanding of mechanisms involved in the development and consequences of pathological calcification, which may uncover strategies to enable treatments for vascular calcification.

Publications

Key publications: 

Furmanik M, Chatrou M, van Gorp R, Akbulut A, Willems B, Schmidt H, van Eys G, Bochaton-Piallat ML, Proudfoot D, Biessen E, Hedin U, Perisic L, Mees B, Shanahan C, Reutelingsperger C, Schurgers L. Reactive Oxygen-Forming Nox5 Links Vascular Smooth Muscle Cell Phenotypic Switching and Extracellular Vesicle-Mediated Vascular Calcification. Circ Res. 2020 Sep 11;127(7):911-927. doi: 10.1161/CIRCRESAHA.119.316159. Epub 2020 Jun 22. PMID: 32564697.

Proudfoot D. Calcium Signaling and Tissue Calcification. Cold Spring Harb Perspect Biol. 2019 Oct 1;11(10):a035303. doi: 10.1101/cshperspect.a035303. PMID: 31138543; PMCID: PMC6771368.

Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release. Yana Dautova, Alexander N. Kapustin, Kevin Pappert, Matthias Epple, Hanneke Okkenhaug, Simon J. Cook, Catherine M. Shanahan, Martin D. Bootman, Diane Proudfoot. Journal of Molecular and Cellular Cardiology, 2018:115, 82-93.  DOI: https://doi.org/10.1016/j.yjmcc.2017.12.007

Dautova Y, Kozlova D, Skepper JN, Epple M, Bootman MD, Proudfoot D.  Fetuin-A and albumin alter cytotoxic effects of calcium phosphate nanoparticles on human vascular smooth muscle cells. PLoS One. 2014 May 21;9(5):e97565. doi: 10.1371/journal.pone.0097565.

Fetuin-A and albumin alter cytotoxic effects of calcium phosphate nanoparticles on human vascular smooth muscle cells. Dautova Y, Kozlova D, Skepper JN, Epple M, Bootman MD, Proudfoot D. PLoS One. 2014 May 21;9(5):e97565.

Human vascular smooth muscle cell culture. Proudfoot D, Shanahan C. Methods Mol Biol. 2012;806:251-63.

Proudfoot D, Shanahan CM. Nanocrystals seed calcification in more ways than one. Kidney Int. 2011 Feb;79(4):379-82. doi: 10.1038/ki.2010.455.

Calcium phosphate crystals induce cell death in human vascular smooth muscle cells: a potential mechanism in atherosclerotic plaque destabilization. Ewence AE, Bootman M, Roderick HL, Skepper JN, McCarthy G, Epple M, Neumann M, Shanahan CM, Proudfoot D. Circ Res. 2008 Aug 29;103(5):e28-34.

Visiting Scientist - Babraham Institute
Chief Scientific Officer- PlaqueTec Ltd
Dr Diane  Proudfoot

Affiliations

Collaborator profiles: 
Classifications: 
Person keywords: 
vascular smooth muscle cells (VSMC)
Cell Death
Atherosclerosis
calcium signalling
Vascular calcification
cell signalling
Inflammation
Calcium Phosphate Particles
cell death