Research
MR JAMES COOPER HAS LEFT CAMBRIDGE AND WORKS NOW IN PROF CHANDRAN'S GROUP AT THE UNIVERSITY OF EDINBURGH
Prior to my current role, I worked at Wellcome Trust Sanger Institute as an Advanced Research Assistant, expanding and maintaining the International Knockout Mouse Consortium (IKMC) miRNA Knockout Resource (miRKO), with focus on high-throughput vector construction, mouse ES cell culture, targeting, and genotyping.
I moved to the University in March 2014, to provide core support to the newly established BHF Oxbridge Centre of Regenerative Medicine. My primary role has been in Genome Engineering with focus on editing/transgenics using CRISPR/Cas9 - projects I have been involved with so far have been listed below:
- CRISPR/Cas9 reporter knock-ins for hPSC epicardium cell lines (Dr Sanjay Sinha)
- CRISPR/Cas9 system to modify histone methylation at specific gene promoters in human VSMCs (Dr Helle Jørgensen)
- Optimized inducible shRNA and CRISPR/Cas9 platforms to study human development (Prof Ludovic Vallier)
- BMPR2 reporter CRISPR/Cas9 knock-in (Dr Amer Rana)
- Gene activation using CRISPR-on strategy (Dr Helle Jørgensen)
- Non-perturbing Reporter knock-in with CRISPR/Cas9 (Prof Roger Pedersen)
- Gene repair model for CADASIL mutation in hESC using TALENs (Dr Sanjay Sinha)
- Gene repair strategy for TAAD mutation using CRISPR/Cas9 (Dr Sanjay Sinha)
- Epigenetic control of vascular smooth muscle cell plasticity (Dr Helle Jørgensen)
Publications
Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha S, Kotter M, Vallier L. Development. 2016 Dec 1;143(23):4405-4418.
